Lawrence Berkeley National Lab's ( ) Molecular Biophysics & Integrated Bioimaging ( ) Division has an opening for a Biologist Research Scientist to join the team.
The Research Scientist will develop a multimodal imaging research program focused on characterizing microbial plant interactions in the rhizosphere. The project aims to link transmission electron microscopy with correlative fluorescent imaging, FTIR and NanoSIMS. The ultimate aim of the project is to understand how specific plant hormones influence the formation of microbial/plant interfaces and the flow of nutrients/carbon products in the rhizosphere and the nanometer/single cell level. The Research Scientist is expected to actively pursue funding for this project.
What You Will Do:
Establish a strong active collaboration between the Joint Genome Institute ( ), MBIB, and Environmental Genomics and Systems Biology ( ).
Grow plant seedling in EcoFAB with defined bacterial cultures.
Maintain and operate a Jeol1400 TEM and Jeol3200 TEM.
Establish a freeze substitution and resin embedding lab.
Prepare rhizosphere samples for NanoSIMs, FIBSEM, TEM, FTIR, and 3D super-resolution microscopy.
Develop and expand research directions in the multimodal rhizosphere imaging program.
Work independently and accurately document experiments in laboratory notebooks.
Develop and manage health and safety requirements relating to the multimodal rhizosphere imaging program.
Dissemination of research via presentations and publications.
Actively pursue grant funding to support the multimodal rhizosphere imaging program.
Actively participate in IDEA.
What is Required:
Up to 5 years of experience in multimodal imaging of symbiotic interactions and microbial communities beyond the highest degree in a related field.
Experience with TEM operation for negative stain imaging, resin embedding and cryo-imaging.
Experience with stable isotope probing and NanoSIMS, cryo preservation, freeze substitution, ultramicrotomy.
Experience and knowledge of automatic data collection with SerialEM.
Experience working with EcoFAB.
Experience with visualization of host-microbes interactions using fluorescent and confocal microscopy.
Experience with preparing a work plan and control documentation for working with recombinant cell lines.
Experience and evidence of successful grant preparation and funding.
Ability to work independently and operate a JEOL1400 Flash TEM and JEOL3200 TEM.
Ability to independently perform cryo-preservation, freeze substitution, resin embedding and ultramicrotomy of biological samples.
Knowledge of and ability to perform cryotomography data collection and processing.
Knowledge of and ability to perform sterile technique.
Demonstrated ability to obtain grant funding.
Ph.D. in Biology, Biochemistry, or a related field.
This is a full-time career appointment, exempt (monthly paid) from overtime pay.
This position may be subject to a background check. Any convictions will be evaluated to determine if they directly relate to the responsibilities and requirements of the position. Having a conviction history will not automatically disqualify an applicant from being considered for employment.
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Work will be primarily performed at Donner Hall at University of California, Berkeley - Donner Hall, Berkeley 94720-4767
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Molecular Biophysics and Integrated Bioimaging
Mission: To generate a mechanistic and predictive understanding of biological processes, by developing and applying molecular- and meso-scale visualization and advanced spectroscopies, enabling the control, manipulation and generation of biological function.
Vision: To generate fundamental knowledge that inspires a mechanistic understanding of biological processes, with the ultimate goal of manipulating, controlling, and creating biological functions in order to solve national challenges in energy, environment, health and biomanufacturing.
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